▶个人简介:
孔德明,男,1975年05月09日出生,籍贯吉林省,博士,现为南开大学化学系教授。
▶基本资料:
二级学科 分析化学
毕业院校 南开大学
生物化学分析
(1)DNA-Hemin过氧化物模拟酶应用研究
(2)小分子与DNA的相互作用研究。
▶承担科研项目:
1. 荧光偏振基因探针及其在肿瘤诊断中的应用研究,国家自然科学基金(20405007) 23万元 (已结题)
2.金属配合物对端粒酶的调控作用及筛选研究,国家自然科学基金(20675041) 28万元 (2007.1-2009.12)
3. DNA-hemin过氧化物模拟酶体系的构效关系及应用研究,国家自然科学基金(20975055) 34万元 (2010.1-2012.12)
4. 端粒酶活性的特异性定量检测技术及诊断试剂盒的开发,天津市应用基础及前沿技术研究计划(08JCZDJC21200)20万元 (2008.4-2010.4)
▶参与项目:
1. 分子探针技术若干关键问题的研究(2006CB705700) 973项目
2008年获天津市自然科学基金二等奖(第二完成人)
发表SCI收录论文30余篇,影响因子大于3.0的15篇,其中大于5.0的6篇
▶近几年代表性文章:
1.-Ming Kong,* Ya-Wei Jin, Yu-Ji Yin, Huai-Feng Mi, Han-Xi Shen. Real-time PCR detection of telomerase activity using specific molecular beacon probes. Analytical and Bioanalytical Chemistry, 2007, 388, 699-709
2. De-Ming Kong,* Jing Wu, Yong-E Ma, Han-Xi Shen, A new method for the study of G-quadruplex ligands. Analyst 2008, 133, 1158-1160.
3.Ming Kong,* Jiao Wang, Li-Na Zhu, Ya-Wei Jin, Xiao-Zeng Li, Han-Xi Shen, Huai-Feng Mi, Oxidative DNA cleavage by Schiff base tetraazamacrocyclic oxamido nickel(II) complexes. Journal of Inorganic Biochemistry 2008, 102, 824-832.
4. De-Ming Kong,* Yong-E Ma, Jing Wu, Han-Xi Shen,Discrimination of G-quadruplexes with duplex and single-stranded DNAs by fluorescence and energy transfer fluorescence spectra of crystal violet. Chemistry - A European Journal 2009, 15, 901-909.
5. De-Ming Kong*, Yong-E Ma, Jun-Hong Guo, Wei Yang, Han-Xi, Shen, Fluorescent sensor for monitoring structural changesof G-quadruplexes and detection of potassium ion. Analytical Chemistry 2009, 81, 2678-2684.
6. De-Ming Kong,* Li-Li Cai, Jun-Hong Guo, Han-Xi Shen,Characterization of the G-quadruplex structure of a catalytic DNA with peroxidase activity, Biopolymers 2009, 91, 331-339.
7. De-Ming Kong*,Jun-Hong Guo, Jing Wu, Yong-E Ma, Han-Xi Shen, Crystal violet-G-quadruplex complexes as fluorescent sensors for homogeneous detection of potassium ion. Biosensor & Bioelectronics 2009, 25, 88-93.
8. Xue-Hui Zhou, De-Ming Kong*,Han-Xi Shen, Ag+ and Cysteine Quantitation Based on G-Quadruplex-Hemin DNAzymes Disruption by Ag+. Analytical Chemistry, 2010, 1216, 807-814.